- Title
- Type 2 and type 17 effector cells are increased in the duodenal mucosa but not peripheral blood of patients with functional dyspepsia
- Creator
- Burns, Grace L.; Bruce, Jessica K.; Bollipo, Steven; Foster, Robert; Gan, Lay T.; Shah, Ayesha; Koloski, Natasha A.; Foster, Paul S.; Horvat, Jay C.; Veysey, Martin; Holtmann, Gerald; Powell, Nick; Minahan, Kyra; Walker, Marjorie M.; Talley, Nicholas J.; Keely, Simon; Mathe, Andrea; Fairlie, Thomas; Cameron, Raquel; Naudin, Crystal; Nair, Prema M.; Potter, Michael D. E.; Irani, Mudar Zand
- Relation
- Frontiers in Immunology Vol. 13, no. 1051632
- Publisher Link
- http://dx.doi.org/10.3389/fimmu.2022.1051632
- Publisher
- Frontiers Research Foundation
- Resource Type
- journal article
- Date
- 2022
- Description
- Background: Functional dyspepsia is characterised by chronic symptoms of post-prandial distress or epigastric pain not associated with defined structural pathology. Increased peripheral gut-homing T cells have been previously identified in patients. To date, it is unknown if these T cells were antigen-experienced, or if a specific phenotype was associated with FD. Objective: This study aimed to characterise T cell populations in the blood and duodenal mucosa of FD patients that may be implicated in disease pathophysiology. Methods: We identified duodenal T cell populations from 23 controls and 49 Rome III FD patients by flow cytometry using a surface marker antibody panel. We also analysed T cell populations in peripheral blood from 37 controls and 61 patients. Where available, we examined the number of duodenal eosinophils in patients and controls. Results: There was a shift in the duodenal T helper cell balance in FD patients compared to controls. For example, patients had increased duodenal mucosal Th2 populations in the effector (13.03 ± 16.11, 19.84 ± 15.51, p=0.038), central memory (23.75 ± 18.97, 37.52 ± 17.51, p=0.007) and effector memory (9.80±10.50 vs 20.53±14.15, p=0.001) populations. Th17 populations were also increased in the effector (31.74±24.73 vs 45.57±23.75, p=0.03) and effector memory (11.95±8.42 vs 18.44±15.63, p=0.027) subsets. Peripheral T cell populations were unchanged between FD and control. Conclusion: Our findings identify an association between lymphocyte populations and FD, specifically a Th2 and Th17 signature in the duodenal mucosa. The presence of effector and memory cells suggest that the microinflammation in FD is antigen driven.
- Subject
- functional dyspepsia; T cells; lymphocytes; immunology; functional gastrointestinal disorder (FGID); SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1481403
- Identifier
- uon:50715
- Identifier
- ISSN:1664-3224
- Rights
- © 2023 Burns, Bruce, Minahan, Mathe, Fairlie, Cameron, Naudin, Nair, Potter, Irani, Bollipo, Foster, Gan, Shah, Koloski, Foster, Horvat, Veysey, Holtmann, Powell, Walker, Talley and Keely. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) http://creativecommons.org/licenses/by/4.0/. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Language
- eng
- Full Text
- Reviewed
- Hits: 1795
- Visitors: 1841
- Downloads: 55
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 10 MB | Adobe Acrobat PDF | View Details Download |